Coding

Part:BBa_K4759047:Design

Designed by: Shiyue Zhao   Group: iGEM23_Jiangnan-China   (2023-10-05)


fdr_0978-RBS2-fdx_1499


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1219
    Illegal PstI site found at 281
    Illegal PstI site found at 607
    Illegal PstI site found at 922
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1219
    Illegal PstI site found at 281
    Illegal PstI site found at 607
    Illegal PstI site found at 922
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1219
    Illegal BglII site found at 1037
    Illegal BamHI site found at 1213
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1219
    Illegal PstI site found at 281
    Illegal PstI site found at 607
    Illegal PstI site found at 922
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1219
    Illegal PstI site found at 281
    Illegal PstI site found at 607
    Illegal PstI site found at 922
    Illegal NgoMIV site found at 625
    Illegal AgeI site found at 697
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1342


Design Notes

Connect fdr_0978 and fdx_1499 via RBS2 for transcription under the control of the same promoter


Source

Ferredoxin reductase

References